Enzymatic synthesis of fructooligosaccharides from inulin in a batch system

Enzymatic synthesis of fructooligosaccharides from inulin in a batch system

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Inulin is Pedestal a robust substrate for the preparation of fructooligosaccharides.In the present research, hydrolysis parameters were optimized for the preparation of fructooligosaccharides from inulin using Aspergillus tritici endoinulinase in a batch system.Under optimized hydrolytic conditions viz.inulin concentration 6%, endoinulinase load 50 IU, hydrolysis time 10 h and agitation 125 rpm, 97.

27% fructooligosaccharides yield was obtained.Fructooligosaccharides preparation contained 33.16% Hairball Remedy ketose (GF2), 24.00% nystose (GF3) and 7.

39% fructofuranosylnystose (GF4).High substrate concentration, low enzyme dose and short hydrolytic period justifies the efficiency of the developed bioprocess for fructooligosaccharides synthesis from inulin by fungal inulinase.